[Tool] Transcriptomic Tools for Studying Cassava Bacterial Blight
Cassava bacterial blight is a major constraint on cassava productivity worldwide. Collaborating with researchers from Hainan University and the National Key Laboratory for Tropical Crop Breeding, we identified stable reference genes for RT-qPCR analysis of cassava responses to infection by Xanthomonas phaseoli. Two genes, MehnRNPR and MePRPF38B, demonstrated superior expression stability across resistant and susceptible varieties. These validated reference genes improve the accuracy of gene expression studies investigating host–pathogen interactions. The work provides essential molecular tools for identifying genes associated with disease resistance and immune responses in cassava.
Media Resources
Yang, Jing, Ciyun Li, Jie Chen, Dongying Lu, Qi Yang, Ruotong Li, Liyun Yang, Xiaofei Zhang, Yinhua Chen, Shousong Zhu, and et al. 2025. "Transcriptome-Based Identification of Reference Genes for Expression Analysis in Cassava Under Xanthomonas phaseoli pv. manihotis Infection" Plants 14, no. 23: 3655. https://doi.org/10.3390/plants14233655