Biofortified cassava varieties enriched in provitamin A are critical for addressing micronutrient deficiencies in tropical regions. In collaboration with scientists from IITA Nigeria, CIAT, Texas A&M, the University of Hawaii, Cornell University, and the IITA Zambia hub, this study evaluated the effectiveness of KASP and DArTseq marker systems for diversity analysis, quality control, and trait selection in biofortified cassava breeding populations.